LncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating miR-144-3p/EZH2 axis

LncRNA NEAT1 in endometrial cancer

Abstract

Background: Endometrial cancer (EC) was one of the most common gynaecological tumors in the worldwide. Long non-coding RNAs (lncRNAs) nuclear enriched abundant transcript 1 (NEAT1) promotes cell proliferation, migration and invasion in EC cell. However, the molecular mechanisms of NEAT1 in EC has not been fully clarified. We conducted this study to reveal the function of NEAT1 in EC tissues and cell lines.

Methods: The cancer tissues and adjacent tissues of EC patients were collected. HEC-1A and Ishikawa cells were cultured in vitro. The expression of NEAT1 was downregulated by transfecting small hairpin RNA (shRNA) and miR-144-3p was overexpressed by transfecting miR-144-3p mimics. Cell proliferation was detected by MTT assay and colony formation assay. Cell migration and invasion ability was assessed by transwell assay. Dual-luciferase reporter assay was used to verify the relationship between  NEAT1, EZH2, and miR-144-3p. The expression of EZH2 was measured by Western blot and qPCR.

Results: NEAT1 was highly expressed in EC tissues and cells. Knockdown of NEAT1 inhibited the proliferation, migration and invasion of EC cells. Additionally, NEAT1 act as a ceRNA of miR-144-3p, leading to the upregulation of EZH2. Overexpression of miR-144-3p suppressed the proliferation and invasion of EC cells.

Conclusion: NEAT1 promotes endometrial cancer proliferation and invasion by regulating miR-144-3p/EZH2 axis.