EFFECT OF IONIZING RADIATION ON HUMAN SKELETAL MUSCLE PRECURSOR CELLS

  • Tomaz Mars University of Ljubljana, Faculty of Medicine, Institute of Pathophysiology

Abstract

Maintenanceof skeletal muscle mass and function is dependent on process of muscleregeneration and ability of satellite cells to activate into proliferativemyoblasts. Following irradiation, number ofsatellite cells available for proliferation, fusion and formation of myotubesmight be insufficient due to impairedactivation, proliferation,cytokine signalling and finally differentiation caused by exposure toirradition. In this study we aimed to explore the acute andlong-term effects of therapeutic doses of ionizing radiation on human myoblastsproliferation, cytokine signalling and stress response capacity. Myoblastscultures have been irradiated by Darpac 2000 X-ray unit at doses of 4, 6 and 8Gy. Compared with non-irradiated control and cells treated with inhibitor ofcell proliferation Ara C, myoblast proliferation decreased 72 hpost-irradiation, this effect more pronounced with increasing doses.Post-irradiation myoblast survival determined by released LDH enzyme activity measurement revealedincreased activity when exposed to irradiation. Acute response of myoblasts to lower doses ofirradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6Higherdose of irradiation triggered stress response in myoblasts with increased levelsof stress markers (heat shock protein 27 and 70). Based on results obtained we conclude that myoblastare responsive and sensitive to irradition in dose-dependent manner in regardto their proliferation capacity and capacity to secret IL-6. Since myoblastproliferation and differention represent key stage in muscle regeneration, thiseffect of irradition need to be taken in account particulary in some clinicalcondition.

Author Biography

Tomaz Mars, University of Ljubljana, Faculty of Medicine, Institute of Pathophysiology
Published
2013-10-23
How to Cite
Mars, T. (2013). EFFECT OF IONIZING RADIATION ON HUMAN SKELETAL MUSCLE PRECURSOR CELLS. Radiology and Oncology, 47(4). Retrieved from https://radioloncol.com/index.php/ro/article/view/1900
Section
Experimental radiology