Proteomic Analysis of Effects by X-rays and Heavy ion in HeLa Cells

  • Zhitong Bing Institute of Modern Physics, Chinese Academy of Sciences
  • Guanghui Yang Institute of Modern Physics, Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences
  • Fengling Wang Biochemistry and molecular laboratory, Medical College of Henan University
  • Yanan Zhang Institute of Modern Physics, Chinese Academy of Sciences
  • Caiyong Ye Institute of Modern Physics, Chinese Academy of Sciences
  • Jintu Sun Institute of Modern Physics, Chinese Academy of Sciences
  • Guangming Zhou Institute of Modern Physics, Chinese Academy of Sciences
  • Lei Yang Institute of Modern Physics, Chinese Academy of Sciences

Abstract

Background

Carbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistance cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradiation at 1 h by a clinically relevant radiation dose (2 Gy X-ray and carbon beam). The differential expression proteins were collected for analysing biological effects.

Materials and Method

The radioresistance cell lines Hela cells were used. In our study, the stable isotope labelling with amino acids (SILAC) method coupling with 2D-LC-LTQ Orbitrap mass spectrometry were applied to identity and quantify the differentially expressed proteins after irradiation. The Western blotting experiment was using to validate the data.

Results

A total of 123 and 155 significantly changed proteins were evaluated with treatment of 2 Gy carbon and X-rays after radiation 1 h, respectively. These deregulated proteins were found to be mainly involved in several kinds of metabolism processes through Gene Ontology (GO) enrichment analysis.

Conclusions

The radioresistance of  the cancer cells treated with 2 Gy X-rays irradiation is largely due to glycolysis enhancement, while the greater killing effect of 2 Gy carbon is due to decreased amino acid metabolism.

Published
2014-05-09
How to Cite
Bing, Z., Yang, G., Wang, F., Zhang, Y., Ye, C., Sun, J., Zhou, G., & Yang, L. (2014). Proteomic Analysis of Effects by X-rays and Heavy ion in HeLa Cells. Radiology and Oncology, 48(2). Retrieved from https://radioloncol.com/index.php/ro/article/view/1957
Section
Experimental radiology