Proteomic Analysis of Effects by X-rays and Heavy ion in HeLa Cells
Abstract
BackgroundCarbon ion therapy may be better against cancer than the effects of a photon beam. To investigate a biological advantage of carbon ion beam over X-rays, the radioresistance cell line HeLa cells were used. Radiation-induced changes in the biological processes were investigated post-irradiation at 1 h by a clinically relevant radiation dose (2 Gy X-ray and carbon beam). The differential expression proteins were collected for analysing biological effects.
Materials and MethodThe radioresistance cell lines Hela cells were used. In our study, the stable isotope labelling with amino acids (SILAC) method coupling with 2D-LC-LTQ Orbitrap mass spectrometry were applied to identity and quantify the differentially expressed proteins after irradiation. The Western blotting experiment was using to validate the data.
ResultsA total of 123 and 155 significantly changed proteins were evaluated with treatment of 2 Gy carbon and X-rays after radiation 1 h, respectively. These deregulated proteins were found to be mainly involved in several kinds of metabolism processes through Gene Ontology (GO) enrichment analysis.
ConclusionsThe radioresistance of the cancer cells treated with 2 Gy X-rays irradiation is largely due to glycolysis enhancement, while the greater killing effect of 2 Gy carbon is due to decreased amino acid metabolism.
- List of all deregulated proteins underlying 2 Gy carbon and X-ray.
- Biological analysis of deregulated proteins by PANTHER system.
- Brief SILAC experiment workflow.
- Distribution of log2 transformed protein expression ratios of two irradiation types.
- The biological process analysis for 2 Gy carbon and 2 Gy X-ray deregulated proteins.
- The highest score network post-irradiation by 2 Gy carbon using IPA analysis.
- The highest score network post-irradiation by 2 Gy X-ray using IPA analysis.
- The highest score network of overlap deregulated proteins underlying 2 Gy carbon and X-ray using IPA analysis.
- The western blotting for analysis of LDHA and SCO1
- SLO abstract
- Supplementary file 1, 2
- Figure 1
- Figure 2
- Figure 3
- Figure 4A
- Figure 4B
- Figure 5
- Figure 7
- Figure 4
- Figure 6
- Supplementary file 3
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